0.1M Potassium Phosphate Buffer, pH7.2

0.1 M Potassium Phosphate Buffer pH 7.2 Solution is widely used in molecular biology, microbiology, biochemistry, biotechnology and tissue culture as dilution and wash buffer as it is isotonic and non?toxic to living cells. It is also used as a wash buffer for immunological assays like western blot, immunohistochemistry, immunofluorescence microscopy and ELISA. It is also used for diluting antibodies during immunological assays or for reagent preparation. It is suitable for general use. 0.1 M Potassium Phosphate Buffer pH 7.2 is a commonly used phosphate buffer, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate. This solution is extensively used for dilution and washing during various biological applications as it maintains the physiological pH and osmolarity. This buffer is developed to provide a standardized medium for the preparation of sample dilutions. It eliminates the variations in pH associated with the use of distilled water. Phosphates have a very high buffering capacity and are highly soluble in water. The phosphate groups of this buffer help to maintain a constant pH