5X Tris-Glycine-SDS Gel Running Buffer

Running buffer for SDS-PAGE of 5X concentration composed of Tris, Glycine and SDS 5X Tris-Glycine-SDS Gel Running Buffer composed of 0.5 M Tris, 1.92 M Glycine, 0.5% SDS is the most commonly used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). This buffer is used as both anode and cathode buffer