Alkaline Transfer Buffer

Alkaline Transfer Buffer is mainly used during Southern blotting procedure where DNA is transferred from agarose gel to positively charged nylon membranes. This buffer comes as a ready to use solution and can be directly used in DNA transfer. Alkaline Transfer Buffer is mainly used to transfer DNA from agarose gels to charged nylon membranes by capillary method during Southern hybridization method. Alkaline Transfer Buffer contains a strong base that denatures double-stranded DNA. This buffer allows the hydrolysis of the phosphodiester backbone at the sites of depurination during Southern blotting and as a result allowing large DNAs to be very efficiently transferred and covalently linked to the positively charged nylon membrane.