Basal Medium Eagle (BME) w/ Hanks' salts and L-Glutamine w/o Sodium bicarbonate

Basal Medium developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. AT001 is Basal Medium Eagle with Earle's balanced salts and L-glutamine. The medium was initially developed as a result of studies to determine the nutritional requirements of HeLa cells and mouse fibroblast L cells in culture. Basal Medium Eagle when properly supplemented supports growth of variety of diploid or primary mammalian cell cultures.