Denhardt’s Solution (100X)

Denhardt's Solution (100X) is a mixture of high molecular weight polymers and is used extensively for blocking non-specific binding sites during membrane-based nucleic acid hybridization protocols. This solution is supplied as 100X concentrate. Denhardt's Solution (100X) is composed of blocking agents This solution is used in membrane-based nucleic acid hybridization protocols like Southern and Northern blotting. Preincubation of nucleic acid containing filters in this solution prevents nonspecific binding of single-stranded DNA probes. Denhardt's Solution (100X) is composed of 1% Ficoll 400, 1% Polyvinylpyrrolidone (PVP) and 1% Bovine Serum Albumin