Dulbecco's Modified Eagle Medium / Nutrient Mixture F12 Ham DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 Ham with L-glutamine. It does not contain calcium chloride, HEPES buffer and sodium bicarbonate. Dulbecco's Modified Eagle Medium / Nutrient Mixture F12 Ham DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. AT189 is Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 Ham with L-glutamine. It does not contain calcium chloride, HEPES buffer and sodium bicarbonate.