Glasgow's Minimum Essential Medium GMEM w/ L-Glutamine and Tryptose Phosphate Broth w/o Sodium bicarbonate

Glasgow's Minimum Essential Medium is a modification of Basal medium Eagle (BME). AT069 is Glasgow's Minimum Essential Medium with L-glutamine and tryptose phosphate broth. Glasgow's Minimum Essential Medium is a modification of Basal medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells, used for investigating the genetic factors affecting cell competence. AT069 is Glasgow's Minimum Essential Medium with L-glutamine and tryptose phosphate broth.