Glasgow's Minimum Essential Medium is a modification of Basal medium Eagle (BME). AT102 is Glasgow's Minimum Essential Medium with L-glutamine. It does not contain tryptose phosphate broth. Glasgow's Minimum Essential Medium is a modification of Basal medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone13 cells, used for investigating the genetic factors affecting cell competence. AT102 is Glasgow's Minimum Essential Medium with L-glutamine. It does not contain tryptose phosphate broth.