Glasgow's Minimum Essential Medium (GMEM) w/ Sodium bicarbonate and NEAA w/o L-Glutamine, Sodium phosphate and Tryptose Phosphate Broth

Glasgow's Minimum Essential Medium(GMEM) is a modification of Basal Medium Eagle(BME). The medium was originally used to culture BHK-21 clone 13 cells. AL058 contains sodium bicarbonate and non-essential amino acids. It does not contain L-glutamine, sodium phosphate and tryptose phosphate broth. Glasgow's Minimum Essential Medium(GMEM) is a modification of Basal Medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells, used for investigating the genetic factors affecting cell competence. AL058 contains sodium bicarbonate and non-essential amino acids. It does not contain L-glutamine, sodium phosphate and tryptose phosphate broth.