Glasgow’s Minimum Essential Medium (GMEM) w/ Tryptose phosphate broth and Sodium bicarbonate w/o L-Glutamine

Glasgow's Minimum Essential Medium(GMEM) is a modification of Basal Medium Eagle(BME). The medium was originally used to culture BHK-21 clone 13 cells. AL069 contains sodium bicarbonate and tryptose phosphate broth. It does not contain L-glutamine. Glasgow's Minimum Essential Medium(GMEM) is a modification of Basal Medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells, used for investigating the genetic factors affecting cell competence. AL069 contains sodium bicarbonate and tryptose phosphate broth. It does not contain L-glutamine.