Hi-Proof DNA Polymerase (2.5 units/μl)

Thermostable enzyme of approximately 90 kDa from Pyrococcus furiosus ,Ultra pure recombinant protein , Eight times more accurate than Taq DNA polymerase , Highly thermostable – remains 95% active after 2 hours incubation at 95o C. , Generates blunt-end PCR products. ,Incorporates modified nucleotides (E.g.- biotin, deoxygenin, fluroscently-labelled nucleotides) Hi-Proof DNA Polymerase (2.5U/µl) is an extremely thermostable polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. Hi-Proof DNA Polymerase (2.5U/µl) catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction in the presence of Mg2+. It exhibits the 3' to 5' exonuclease (Proofreading) activity that enables the polymerase to correct nucleotide incorporation errors resulting in over 10-fold higher fidelity than possible with Taq DNA Polymerase. High fidelity DNA polymerization is obtained with Hi-Proof DNA Polymerase (2.5U/µl), allowing amplification of DNA up to 10 kb long.