HiPurA® Bacterial RNA Purification Kit

HiPurA® Bacterial RNA Purification Kit provides a fast and easy method for purification of Bacterial RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT‐PCR, Differential display, Expression‐array and expression‐chip analysis and cDNA library construction. This kit simplifies isolation of RNA from different types of bacteria (Gram positive and Gram negative) with spin‐column procedure. The lysis buffer provided in the kit helps in cell disruption and denaturation, samples are centrifuged through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material. Ethanol is added to the cleared lysate, which promotes selective binding of RNA to the HiElute Miniprep Spin column membrane. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the Elution Solution provided with the kit.