Krebs-Ringer Bicarbonate Buffer w/ 1.8 gms Glucose per litre w/o Phenol red, Calcium chloride and Sodium bicarbonate

Krebs-Ringer bicarbonate buffer contains 1.8gms per litre Glucose. It does not contain phenol red, calcium chloride and sodium bicarbonate. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to prevent calcium precipitation. Krebs-Ringer bicarbonate buffer contains 1.8gms per litre Glucose. It does not contain phenol red, calcium chloride and sodium bicarbonate.