LoSera™ medium can be used without prior adaptation and sub cultured using normal procedures. Reduced serum supplementation improves the reproducibility of experimental results by decreasing the variability caused due to undefined serum constituents. It also facilitates down regulation process in bioassays and in purification process of culture products. RSL008G is LoSera™ Minimum Essential Medium Eagle with Earle's salts, L-alanyl-L-glutamine, NEAA and sodium bicarbonate.