Medium 199 (For Suspension Culture) w/ Earle’s salts and L-Glutamine w/o Calcium chloride, Magnesium sulphate and Sodium bicarbonate

AT043 is Medium 199 with Earle’s salts and L-Glutamine. It is modified for use with suspension cells, hence does not contain calcium chloride and magnesium sulphate. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950 for nutritional studies on primary chick embryo fibroblasts. Media 199 are formulated with either Hanks salts or Earle’s salts. The medium when supplemented with serum can be used for growth of a wide variety of cells. Media 199 are presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.