Nitrate Reagent Discs (Twin Pack) 1VL

Nitrate reagent discs are used for detection of nitrate reduction by microorganisms.The test involves detection of the enzyme nitrate reductase which causes the reduction of nitrate in the presence of a suitable electron donor to nitrite, which can be tested by an appropriate colorimetric reagent. Almost all Enterobacteriaceae reduce nitrate. Nitrate reagent discs when placed on suspected colony turn red-pink in case of nitrate reduction (positive reaction), when a drop or two of Part B (Rehydrating fluid) is added to the disc. Escherichia coli ATCC 25922 Positive reaction:red or pink colour formation onaddition of nitrate test reagents. Acinetobacter calcoaceticus ATCC 43498b luxuriant negative reaction