Trypsin-EDTA Solution 1X 0.25% Trypsin Gamma-Irradiated and 0.02% EDTA in Hanks' Balanced Salt Solution w/ Phenol red

This solution contains 0.25% Trypsin and 0.02% EDTA in Hank’s balanced salt solution with phenol red. Trypsin is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells. This product contains 0.25% Trypsin and 0.02% EDTA in Hank’s balanced salt solution with phenol red.