Tryptose Cycloserine Azide Agar Base 500G

Tryptose Cycloserine Azide Agar Base is recommended for enumeration of sulphite reducing anaerobes essentially Clostridia. This medium was later modified by decreasing the concentration of D-cycloserine, sulphite and iron and by the addition of sodium azide. This medium utilizes the selective inhibitory properties of D-cycloserine and an indicator system involving sulphite and iron. Growth of non-mesophilic organisms are suppressed while C. perfringens and related species will reduces the sulphite and form black colonies due to the production of ferrous sulphide.