Waymouth's medium comprises of amino acids, vitamins, the purine base hypoxanthine, salts, and glucose. This medium can be used for culturing many fastidious cell lines and also whole organ culture. AL091A contains L-glutamine and sodium bicarbonate. Waymouth Medium MB 752/1 was developed in 1959 for the cultivation of mouse L cells, NCTC clone 929. This medium is a comparatively simple medium with lesser ingredients than its other synthetic counterparts. The medium comprises of amino acids, vitamins, the purine base hypoxanthine, salts, and glucose. This medium can be used for culturing many fastidious cell lines and also whole organ culture. AL091A contains L-glutamine and sodium bicarbonate.