0.5M CAPS, pH 10.5

0.5M CAPS, pH 10.5 is a buffer extensively used in Molecular Biology for the transfer of proteins on to membranes, to investigate metal-dependent enzymes and widely used in Western and immunoblotting experiments. CAPS is the common name for N-cyclohexyl-3-aminopropanesulfonic acid, a chemical which can be used as buffering agent in biochemistry. CAPS is a zwitterionic buffer useful in the range of pH 7.9- 11.1. 0.5M CAPS, pH 10.5 is a buffer extensively used in Molecular Biology. The high pH of this buffer makes it useful for the transfer of proteins on to membranes. CAPS buffer has very low metal-binding constants and are therefore particularly useful to investigate metal-dependent enzymes. CAPS is a highly water soluble buffering reagent widely used in Western and immunoblotting experiments as well as protein sequencing and identification.