10X Tris-Glycine Buffer, pH 8.3

10X Tris-Glycine Buffer, pH 8.3 is used in native polyacrylamide gel electrophoresis of proteins and during the electrotransfer of protein samples form the polyacrylamide gels to the nitrocellulose membrane. 10X Tris-Glycine Buffer, pH 8.3 is commonly used as a electrophoresis buffer in native polyacrylamide gel electrophoresis as it doesn’t not contains SDS and as a transfer buffer during western blotting. It is composed of 0.25 M Tris and 1.92 M Glycine with pH of 8.3.