Basal Medium Eagle (BME) w/ Hanks' salts, L-Glutamine and 25mM HEPES buffer w/o Sodium bicarbonate

Basal Medium developed by Harry Eagle is a combination of essential nutrients in appropriate concentrations for monolayer cultivation of a wide variety of normal and transformed cells. AT062 is Basal Medium Eagle with Hank's balanced salts, L-glutamine and 25mM HEPES buffer. The medium was initially developed as a result of studies to determine the nutritional requirements of HeLa cells and mouse fibroblast L cells in culture. Basal Medium Eagle when properly supplemented supports growth of variety of diploid or primary mammalian cell cultures.