SFM032L is a animal component-free and chemically defined serum-free CHO medium devoid of L-glutamine, hypoxanthine, thymidine and phenol red. The medium is formulated with 0.1% Pluronic® F-68 to protect against mechanical shear damage. Serum free media are designed to grow a specific cell type or perform a specific application in absence of serum. Unlike serum supplemented media which may be utilized for a broad range of cell types and culture conditions, serum free media are specific. SFM032L is a animal component-free and chemically defined serum-free CHO medium devoid of L-glutamine, hypoxanthine, thymidine and phenol red. The medium is formulated with 0.1% Pluronic® F-68 to protect against mechanical shear damage.