Direct Blood PCR Kit

HiMedia's Direct Blood PCR Kit offers very fast, highly‐specific, direct PCR from a wide range of human and animal whole blood samples, including those preserved with anticoagulants stored at different temperatures from +4°C, ‐20°C, and / or ‐70°C. The use of HiMedia's Taq polymerase enzyme has an increased affinity for template DNA, giving a high PCR product yield from the most challenging templates. The kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield. This kit also contains universal 18S and GAPDH primers. A final concentration upto 10% can be used for Direct Blood PCR Kit. Specific primers can be used for identification of the target of interest by the end user. This kit also contain positive control. Researchers often purify DNA from blood samples prior to performing PCR because it is believed that blood constituents and the reagents commonly used to preserve blood samples (e.g., anticoagulants) interfere with PCR. In order to save the time and expense required for template purification, several methods have been reported for direct PCR of blood samples.HiMedia’s Direct Blood PCR Kit offers very fast, highly-specific, direct PCR from a wide range of human and animal whole blood samples, including those preserved with anticoagulants stored at different temperatures from +4°C, -20°C, and / or -70°C. The use of HiMedia’s Taq polymerase enzyme has an increased affinity for template DNA, giving a high PCR product yield from the most challenging templates.