DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Dulbecco's Modified Eagle Medium is with 1gm glucose per litre, L-glutamine and sodium pyruvate. It does not contain phenol red. Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. AT183 is Dulbecco's Modified Eagle Medium with 1gm glucose per litre, L-glutamine and sodium pyruvate. It does not contain phenol red.