Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. It contains L-glutamine and sodium bicarbonate. It does not contain Glucose and sodium pyruvate. Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. AL269A is Dulbecco's Modified Eagle Medium. It contains L-glutamine and sodium bicarbonate. It does not contain Glucose and sodium pyruvate.