Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. Dulbecco's Modified Eagle Medium contains sodium bicarbonate and sodium pyruvate. It does not contain Glucose and L-glutamine. Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. AL186 is Dulbecco's Modified Eagle Medium. It contains sodium bicarbonate and sodium pyruvate. It does not contain Glucose and L-glutamine.