Gentamicin Solution w/ 10mg/ml Gentamicin sulfate in sterile tissue culture grade water

Geneticin (G418) blocks polypeptide synthesis by inhibiting protein elongation by binding irreversibility to 80S ribosomes both in prokaryotic and eukaryotic cells. This antibiotic solution is sterile filtered containing 10mg gentamicin per ml. Gentamicin is a broad spectrum bacteriocidal agent of aminoglycoside group and is effective against Gram positive bacteria and Gram negative bacteria. It is a mixture of the three major components C1, C1a and C2. Gentamicin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes.