Grace's Insect Medium w/ Sodium bicarbonate w/o L-Glutamine

Grace's Insect medium is a modification of the original formula developed by Wyatt. Grace(1962) improved the medium by introducing ten water soluble vitamins in Wyatt's formula. Grace successfully established four strains of cells from ovarian tissue of Australian emperor gum moth using this medium. IML001 can be used to culture cells derived from a variety of insects especially Lepidopterans and some species of Dipterans. It does not contain L-glutamine. Presently, the medium is supplemented with 5-20% fetal bovine serum. Grace's Insect medium is a modification of the original formula developed by Wyatt. Grace(1962) improved the medium by introducing ten water soluble vitamins in Wyatt's formula. Grace successfully established four strains of cells from ovarian tissue of Australian emperor gum moth using this medium. These were the first continuous cell lines developed from insect tissue. IML001 can be used to culture cells derived from a variety of insects especially Lepidopterans and some species of Dipterans. It does not contain L-glutamine. Presently, the medium is supplemented with 5-20% fetal bovine serum.