Hi-SYBr Master Mix (with Taq Polymerase), supplied in 2X concentration, is a ready-to-use mix convenient for realtime PCR Hi-SYBr Master Mix (with Taq Polymerase), supplied in 2X concentration, is a ready-to-use mix convenient for realtime PCR. The master mix contains: 1.SYBr Green Dye 2.Taq polymerase 3.dNTPs 4.Assay buffer 5.MgCl2 Template, primers and nuclease-free water should be added before setting up the PCR reaction. As the mixture is ready-to-use, the reaction set time is reduced to half.