HiPurA® Endotoxin free Plasmid DNA Miniprep Purification Kit

The kit utilizes an advanced silica-based membrane technology in the form of a convenient spin column facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. HiPurA® Endotoxin free Plasmid DNA Miniprep Purification Kit provides a fast and easy method for purification of endotoxin free plasmid DNA from E.coli host strains using the miniprep spin columns. The kit utilizes an advanced silica-based membrane technology in the form of a convenient spin column facilitating the binding, washing and elution steps thus enabling multiple samples to be processed simultaneously. It comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure plasmid DNA. This kit integrates an efficient endotoxin removal step into plasmid purification procedure. Concentration of DNA can be estimated by agarose gel electrophoresis. Typical DNA yield is upto 20 µg from 1-5ml of cell culture.