MCDB 153 Medium w/ Trace elements, L-Glutamine and 28mM HEPES buffer w/o Sodium bicarbonate

MCDB 153 medium is a modification of Ham's nutrient mixture F-12 designed for the growth of non-transformed cell types in a serum-free formulation. AT135 is MCDB 153 with trace elements, L-glutamine and 28mM HEPES buffer. MCDB media were developed for the low-protein and serum free growth of specific cell types using hormones, growth factors, trace elements and/or low levels of dialyzed fetal bovine serum protein (FBSP). MCDB 153 medium is specific for human epidermal keratinocyte culture, clonal growth of chicken embryo fibroblasts or Chinese Hamster Ovary (CHO) cells using low levels of FBSP.