Nutrient Mixture F-12 Ham, Coon’s Modification w/o L-Glutamine and Sodium bicarbonate

AT146A is Nutrient Mixture F-12 Ham, Coon's modification without L-glutamine. AT146 is allows growth of CHO cells, mouse cells and other mammalian cells with or without serum depending on the cell type. Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. Ham's Nutrient Mixture F12, Coon's modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid.