Nutrient Mixture F-12 Ham, Coon’s Modification w/ Sodium bicarbonate w/o L-Glutamine

Ham’s Nutrient Mixture F12, Coon’s modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid. AL146 is Coon’s modification of Nutrient Mixture F-12 Ham without L-glutamine. Ham's Nutrient Mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L cells. It is the medium of choice for the growth of cells of rodent origin, cloning of myeloma and hybridoma cells and performing clonal toxicity assay using CHO cells. Ham’s Nutrient Mixture F12, Coon’s modification was developed to determine optimal conditions for obtaining viable hybrids by treatment of parental cells with sendai virus. The medium was modified by doubling the concentration of amino acids and pyruvate and adding ascorbic acid. AL146 is Coon’s modification of Nutrient Mixture F-12 Ham without L-glutamine.