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Catalog No.
CS-M282-500G
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PKU Test Agar Base is recommended for estimation of phenylalanine in blood for detection of Phenylketonuria (PKU). Cultural repsonse of Bacillus subtilis in presence of β-2-thienylalanine is studied using this medium. Small filter paper discs saturated with patients blood are placed on PKU Test Agar with b-2-thienylalanine inoculated with Bacillus subtilis along with control discs with different levels of L-phenylalanine. After overnight incubation, zones of growth around the paper discs are compared. A response comparable to 4 mg% control disc is considered as presumptive positive.
