Slanetz and Bartley Medium 500G

Slanetz and Bartley Medium is recommended for detection and enumeration of faecal Streptococci by membrane filter technique. The composition and performance criteria are in accordance with ISO/DIS 7899-2, 1984. The medium is highly selective for Enterococci. Sodium azide inhibits gram-negative organisms. Triphenyl Tetrazolium Chloride is reduced to the insoluble formazan inside the bacterial cell forming dark red-coloured colonies. When the medium is incubated at higher temperature (44-45°C), all red or maroon colonies can be considered as presumptive Enterococci. Enterococcus faecalis grows luxuriantly as red or maroon colonies while Escherichia coli is inhibited on this medium.