SM Buffer

SM Buffer is mainly used for routine manipulation of phage suspensions. It is used for preparation and storage of lamda phage. SM Buffer is a mixture of Sodium chloride, Magnesium sulphate and gelatin and is extensively used in molecular biology. It contains 100mM Sodium chloride, 10mM Magnesium sulphate, 50mM Tris-HCl, pH 7.5 and 0.01% (w/v) gelatin. This is used as a phage diluent and storage buffer as the gelatin stabilizes lambda phage