STET Buffer

STET Buffer is a mixture of Sodium chloride, Tris-Cl, EDTA and Triton X-100 used for lysis of bacterial cells during nucleic acid isolation and protein purification procedure. STET Buffer is a mixture of Sodium chloride, Tris-Cl, EDTA and Triton X-100. It is used in biomolecular procedures. It is extensively used to lyse E. coli cells rapidly and efficiently during plasmid DNA extraction, RNA isolation or protein purification procedures. It is is composed of 10mM Tris-HCl, 1mM EDTA, 100mM NaCl and 5% Triton* X- 100.