TNM-FH Insect Medium w/ Lactalbumin hydrolysate, Yeast extract and Sodium bicarbonate w/o L-Glutamine

TNM-FH medium is a modification of Grace's insect medium. The modification was developed by Dr. W. F. Hink to grow Cabbage looper cell line TN-368. Presently, this medium is used to grow cells derived from a variety of lepidopterans species. IML008 is supplemented with lactalbumin hydrolysate and yeast extract. It does not contain L-glutamine. Lactalbumin hydrolysate provides a large number of free amino acids. Yeast extract serves as a source of vitamins. Supplemented with 5-20% fetal bovine serum this medium supports growth of number of insect cell lines derived from Lepidopteran species. TNM-FH medium is a modification of Grace's insect medium. The modification was developed by Dr. W. F. Hink to grow Cabbage looper cell line TN-368. Presently, this medium is used to grow cells derived from a variety of lepidopterans species. IML008 is supplemented with lactalbumin hydrolysate and yeast extract. It does not contain L-glutamine. Lactalbumin hydrolysate provides a large number of free amino acids. Yeast extract serves as a source of vitamins. Supplemented with 5-20% fetal bovine serum this medium supports growth of number of insect cell lines derived from Lepidopteran species.